The cryo-EM structure of the endocytic receptor DEC-205.
Benjamin S Gully, Hariprasad Venugopal, Alex J Fulcher, Zhihui Fu, Jessica Li, Felix A Deuss, Carmen Llerena, William R Heath, Mireille H Lahoud, Irina Caminschi, Jamie Rossjohn, Richard Berry
Journal of Biological Chemistry | American Society for Biochemistry & Molecular Biology (ASBMB) | Published : 2020
DEC-205 (CD205), a member of the macrophage mannose receptor protein family, is the prototypic endocytic receptor of dendritic cells, whose ligands include phosphorothioated cytosine-guanosine (CpG) oligonucleotides, a motif often seen in bacterial or viral DNA. However, despite growing biological and clinical significance, little is known about the structural arrangement of this receptor or any of its family members. Here we describe the 3.2 Å cryo-EM structure of human DEC-205, thereby illuminating the structure of the mannose receptor protein family. The DEC-205 monomer forms a compact structure comprising two intercalated rings of C-type lectin-like domains, where the N-terminal cysteine..View full abstract