Journal article

Odontoblast markers and dentine reactions in carious primary molars with and without hypomineralised enamel defects

Janita Shah, David J Manton, Michael J McCullough, Sadna Rajan

International Journal of Paediatric Dentistry | WILEY | Published : 2020


BACKGROUND: Wnt/β-Catenin signalling and DMP1 have key roles in tertiary dentinogenesis. AIM: To compare the relationship between remaining dentine thickness (RDT), tertiary dentine thickness (TDT), β-catenin and dentine matrix protein 1 (DMP1) in carious second primary molar teeth with normal (SPM) and hypomineralised enamel (HSPM). DESIGN: Extracted carious SPM and HSPM were fixed, sectioned (5 μm) and stained with haematoxylin and eosin or with indirect immunofluorescence for β-catenin and DMP1. Image analysis was performed to determine RDT, TDT, β-catenin and DMP1 intensity in the odontoblast layer and dentine-pulp complex. RESULTS: Carious SPM (n = 11; mean RDT = 1536.1 μm) and HSPM (n ..

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University of Melbourne Researchers