Journal article

A comparison of non-magnetic and magnetic beads for measuring IgG antibodies against Plasmodium vivax antigens in a multiplexed bead-based assay using Luminex technology (Bio-Plex 200 or MAGPIX)

Ramin Mazhari, Jessica Brewster, Rich Fong, Caitlin Bourke, Zoe SJ Liu, Eizo Takashima, Takafumi Tsuboi, Wai-Hong Tham, Matthias Harbers, Chetan Chitnis, Julie Healer, Maria Ome-Kaius, Jetsumon Sattabongkot, James Kazura, Leanne J Robinson, Christopher King, Ivo Mueller, Rhea J Longley

PLOS ONE | PUBLIC LIBRARY SCIENCE | Published : 2020

Abstract

Multiplexed bead-based assays that use Luminex® xMAP® technology have become popular for measuring antibodies against proteins of interest in many fields, including malaria and more recently SARS-CoV-2/COVID-19. There are currently two formats that are widely used: non-magnetic beads or magnetic beads. Data are lacking regarding the comparability of results obtained using these two types of beads, and for assays run on different instruments. Whilst non-magnetic beads can only be run on flow-based instruments (such as the Luminex® 100/200™ or Bio-Plex® 200), magnetic beads can be run on both these and the newer MAGPIX® instruments. In this study we utilized a panel of purified recombinant Pla..

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Grants

Awarded by National Health and Medical Research Council Australia


Awarded by National Institute of Allergy and Infectious Diseases (NIH)


Awarded by Global Health Innovative Technology Fund


Awarded by NHMRC Early Career Investigator Fellowship


Funding Acknowledgements

This work was supported by the National Health and Medical Research Council Australia (https://www.nhmrc.gov.au/) (#1092789, #1134989 and #1043345 to IM and #1143187 to W-HT), the National Institute of Allergy and Infectious Diseases (https://www.niaid.nih.gov/grants-contracts/opportunities) (NIH grant 5R01 AI 104822 to JS) and the Global Health Innovative Technology Fund (https://www.ghitfund.org/) (T2015-142 to IM). Additional funding directly supporting field studies was from the TransEPI consortium (supported by the Bill and Melinda Gates Foundation, https://www.gatesfoundation.org/).RJL is currently supported by an NHMRC Early Career Investigator Fellowship (1173210). W. H.T. is a Howard Hughes Medical InstituteWellcome Trust International Research Scholar (https://www.hhmi.org/programs/biomedicalresearch/international-programs, 208693/Z/17/Z). We also acknowledge support from the National Research Council of Thailand. This work was made possible through Victorian State Government Operational Infrastructure Support and Australian Government NHMRC IRIISS. These funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Additional support for this study was provided by CellFree Sciences in the form of salary for MH. The specific roles of these authors are articulated in the `author contributions' section.