RNF41 regulates the damage recognition receptor Clec9A and antigen cross-presentation in mouse dendritic cells
Kirsteen M Tullett, Peck Szee Tan, Hae-Young Park, Ralf B Schittenhelm, Nicole Michael, Rong Li, Antonia N Policheni, Emily Gruber, Cheng Huang, Alex J Fulcher, Jillian C Danne, Peter E Czabotar, Linda M Wakim, Justine D Mintern, Georg Ramm, Kristen J Radford, Irina Caminschi, Meredith O'Keeffe, Jose A Villadangos, Mark D Wright Show all
ELIFE | ELIFE SCIENCES PUBLICATIONS LTD | Published : 2020
The dendritic cell receptor Clec9A facilitates processing of dead cell-derived antigens for cross-presentation and the induction of effective CD8+ T cell immune responses. Here, we show that this process is regulated by E3 ubiquitin ligase RNF41 and define a new ubiquitin-mediated mechanism for regulation of Clec9A, reflecting the unique properties of Clec9A as a receptor specialized for delivery of antigens for cross-presentation. We reveal RNF41 is a negative regulator of Clec9A and the cross-presentation of dead cell-derived antigens by mouse dendritic cells. Intriguingly, RNF41 regulates the downstream fate of Clec9A by directly binding and ubiquitinating the extracellular domains of Cle..View full abstract
National Health and Medical Research Council Peter E Czabotar Linda M Wakim Justine D Mintern Kristen Radford Irina Caminschi Meredith O'Keeffe Jose Villadangos Mark Wright Marnie E Blewitt William Heath Ken Shortman Anthony W Purcell Nicos A Nicola Jian-Guo Zhang Mireille H Lahoud Cancer Council Victoria Kirsteen M Tullett The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.