Journal article

Hypotonic osmotic shock treatment to enhance lipid and protein recoveries from concentrated saltwater Nannochloropsis slurries

Ronald Halim, Ioannis Papachristou, Christin Kubisch, Natalja Nazarova, Ruediger Wuestner, David Steinbach, George Q Chen, Huining Deng, Wolfgang Frey, Clemens Posten, Aude Silve

FUEL | ELSEVIER SCI LTD | Published : 2021


This study reports the use of hypotonic osmotic shock as a treatment step to enhance the recoveries of biofuel-convertible lipids and proteins from lipid-rich saltwater Nannochloropsis gaditana (N. gaditana) slurries (biomass content = ~140 mg biomass / g slurry, total lipid content = ~600 mg lipid /g biomass). The osmotic shock was induced through repeated washing of microalgal slurries with multiple batches of fresh water. Subjecting the slurries to 2 stages of freshwater washing resulted in a measurable damage to cell membranes (the uptake of membrane permeability marker increased by 6 folds), a partial loss of cell viability (only 64% of available cells were recoverable), and a minor rel..

View full abstract

University of Melbourne Researchers


Awarded by Alexander von Humboldt Foundation (Germany)

Awarded by Science and Industry Endowment Fund (Australia)

Funding Acknowledgements

RH is grateful for the generous supports received from the following funding sources: 1) Alexander von Humboldt Postdoctoral Research Fellowship 2017 - 2018 (Ref 3.1-AUS-1192825-HFST-P) from the Alexander von Humboldt Foundation (Germany) and 2) John Stocker Postdoctoral Research Fellowship 2016 - 2020 (PFl6-164) from the Science and Industry Endowment Fund (Australia).