Journal article

Eukaryote-conserved histone post-translational modification landscape in Giardia duodenalis revealed by mass spectrometry

Samantha J Emery-Corbin, Joshua J Hamey, Balu Balan, Laura Rojas-Lopez, Staffan G Svard, Aaron R Jex

INTERNATIONAL JOURNAL FOR PARASITOLOGY | ELSEVIER SCI LTD | Published : 2021

Abstract

Diarrheal disease caused by Giardia duodenalis is highly prevalent, causing over 200 million cases globally each year. The processes that drive parasite virulence, host immune evasion and transmission involve coordinated gene expression and have been linked to epigenetic regulation. Epigenetic regulatory systems are eukaryote-conserved, including in deep branching excavates such as Giardia, with several studies already implicating histone post-translational modifications in regulation of its pathogenesis and life cycle. However, further insights into Giardia chromatin dynamics have been hindered by a lack of site-specific knowledge of histone modifications. Using mass spectrometry, we have p..

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University of Melbourne Researchers

Grants

Awarded by Jack Brockhoff Foundation Early Career Grant, Australia


Awarded by NHMRC Career Development Fellowship


Funding Acknowledgements

This work, including the efforts of SJE, was funded by a Jack Brockhoff Foundation Early Career Grant, Australia (ID JBF 4184, 2016) . SJE, BB and AJ are supported by the Victorian State? Government Operational Infrastructure Support, Australia and the Australian Government National Health and Medical Research Council (NHMRC) Independent Research Institute Infrastructure Support Scheme. AJ is supported by an NHMRC Career Development Fellowship (APP1126395) . The authors also acknowledge the Melbourne Mass Spectrometry and Proteomics Facility of The Bio21 Molecular Science and Biotechnology Institute at The University of Melbourne, Australia, including Shuai Nie, Chingseng Ang and Nick Williamson, for the support of mass spectrometry analysis.