Journal article

Methylation dependent gold adsorption behaviour identifies cancer derived extracellular vesicular DNA

Abu Ali Ibn Sina, Ting-Yun Lin, Ramanathan Vaidyanathan, Zhaoran Wang, Shuvashis Dey, Jing Wang, Andreas Behren, Alain Wuethrich, Laura G Carrascosa, Matt Trau

NANOSCALE HORIZONS | ROYAL SOC CHEMISTRY | Published : 2020

Abstract

Extracellular vesicles (EV) play a major role in intercellular communication by transmitting cellular materials (e.g. protein, RNA) among distant cells. Recent evidence suggests that they could also contribute to carrying DNA which could inform on the mutational status of the parent tumour DNA. Thus, the fundamental analysis of evDNA could open a better understanding of tumour metastasis and provide new pathways for noninvasive detection and monitoring of cancer. To explore the potential of evDNA for diagnostics, the isolation of pure evDNA from body fluids free of cfDNA contamination is crucial. Herein, we use a liposome based model system to develop an improved evDNA isolation protocol fre..

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University of Melbourne Researchers

Grants

Awarded by NHMRC Investigator Grant


Awarded by UQ ECR Grant


Awarded by ARC DP


Funding Acknowledgements

This work was supported by NHMRC Investigator Grant (APP1175047) for AAIS, UQ ECR Grant (UQECR1945613) and ARC DP (DP180102836). These grants have significantly contributed to the environment to stimulate the research described here. A. W. is supported by an NHMRC Investigator Grant (APP1173669). A. B. is supported by a fellowship from the Department of Health and Human Services acting through the Victorian Cancer Agency. The fabrication works were conducted at the Queensland node of the Australian National Fabrication Facility (Q-ANFF).