Journal article

Tracking extracellular vesicle phenotypic changes enables treatment monitoring in melanoma

Jing Wang, Alain Wuethrich, Abu Ali Ibn Sina, Rebecca E Lane, Lynlee L Lin, Yuling Wang, Jonathan Cebon, Andreas Behren, Matt Trau

Science Advances | AMER ASSOC ADVANCEMENT SCIENCE | Published : 2020

Abstract

Monitoring targeted therapy in real time for cancer patients could provide vital information about the development of drug resistance and improve therapeutic outcomes. Extracellular vesicles (EVs) have recently emerged as a promising cancer biomarker, and EV phenotyping shows high potential for monitoring treatment responses. Here, we demonstrate the feasibility of monitoring patient treatment responses based on the plasma EV phenotypic evolution using a multiplex EV phenotype analyzer chip (EPAC). EPAC incorporates the nanomixing-enhanced microchip and the multiplex surface-enhanced Raman scattering (SERS) nanotag system for direct EV phenotyping without EV enrichment. In a preclinical mode..

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University of Melbourne Researchers

Grants

Awarded by National Breast Cancer Foundation of Australia


Awarded by Australian Research Council


Awarded by Swiss National Science Foundation


Awarded by UQ Development Fellowships


Awarded by NHMRC Peter Doherty Biomedical Fellowship


Awarded by Department of Health and Human Services through the Victorian Cancer Agency (VCA)


Funding Acknowledgements

We acknowledge funding from the National Breast Cancer Foundation of Australia (CG-12-07) and the Australian Research Council (DP160102836) to M.T. and Y.W. A.W. is supported by the Swiss National Science Foundation (P2SKP2_168309) and the UQ Development Fellowships (UQFEL1831057). L.L.L. is supported by the NHMRC Peter Doherty Biomedical Fellowship (APP1111216). A.B. is supported by a fellowship (MCRF17019) from the Department of Health and Human Services acting through the Victorian Cancer Agency (VCA). J.W. and R.E.L. acknowledge support from the Australian Government Research Training Program Scholarships.