Journal article
TINC— A Method to Dissect Regulatory Complexes at Single-Locus Resolution— Reveals an Extensive Protein Complex at the Nanog Promoter
AS Knaupp, M Mohenska, MR Larcombe, E Ford, SM Lim, K Wong, J Chen, J Firas, C Huang, X Liu, T Nguyen, YBY Sun, ML Holmes, P Tripathi, J Pflueger, FJ Rossello, J Schröder, KC Davidson, CM Nefzger, PP Das Show all
Stem Cell Reports | CELL PRESS | Published : 2020
Abstract
Here, Knaupp and colleagues describe TINC, an epigenetic method that allows interrogation of mammalian regulatory complexes at a single-locus resolution. TINC was applied to dissect the transcriptional complex at the Nanog promoter in embryonic stem cells, revealing hundreds of interactors, including RCOR2, hence redefining how this gene is regulated in pluripotency.
Grants
Awarded by State Government of Victoria
Funding Acknowledgements
This work was supported by a National Health and Medical Research Council (NHMRC) grant (APP1069830) to R.L. and J.M.P. J.M.P. and R.L. were supported by the Australian Research Council (ARC) Stem Cells Australia Special Initiative, an NHMRC CDF (APP1036587 to J.M.P.), an ARC Future Fellowship (FT120100862 to R.L. and FT180100674 to J.M.P.), and a Silvia and Charles Viertel Senior Medical Research Fellowship. R.L. was also supported by the ARC Center of Excellence program in Plant Energy Biology (CE140100008), HHMI International Research Scholarship, and NHMRC Investigator grant GNT1178460. A.S.K. was supported by an NHMRC ECF (APP1092280). We thank Flowcore, the MHTP Medical Genomics Facility, Micromon, the Monash Bioinformatics and Histology Platforms. This study used BPA-enabled/NCRIS-enabled infrastructure located at the Monash Proteomics and Metabolomics Facility led by R.B.S. The Australian Regenerative Medicine Institute is supported by grants from the State Government of Victoria and the Australian Government. The PiggyBac vector and the OKMS MEFs were kindly provided by Andras Nagy.