Journal article

Porphyromonas gingivalis laboratory strains and clinical isolates exhibit different distribution of cell surface and secreted gingipains

Christine A Seers, A Sayeed M Mahmud, N Laila Huq, Keith J Cross, Eric C Reynolds

JOURNAL OF ORAL MICROBIOLOGY | TAYLOR & FRANCIS LTD | Published : 2021

Abstract

Background: The cell-surface cysteine proteinases RgpA, RgpB (Arg-gingipain), and Kgp (Lys-gingipain) are major virulence factors of P. gingivalis, a keystone pathogen in the development of destructive periodontal disease. The gingipains function as proteinases and transpeptidases utilising small peptides such as glycylglycine as acceptor molecules. However, the characteristics of the gingipains from most P. gingivalis strains have not been determined. Methods: We determined the phenotypes of a panel of P. gingivalis laboratory strains and global clinical isolates with respect to growth on blood agar plus whole-cell and vesicle-free culture supernatant (VFSN) Arg- and Lys-specific proteinase..

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Grants

Awarded by Australian Government Department of Industry, Innovation and Science Grant


Awarded by National Health & Medical Research Council grant


Awarded by Endeavour Foundation Postgraduate Scholarship


Awarded by Australian Dental Research Foundation grant


Funding Acknowledgements

This study was supported by the Australian Government Department of Industry, Innovation and Science Grant 20080108, the National Health & Medical Research Council grant 1081252, an Endeavour Foundation Postgraduate Scholarship 4588_2015, and an Australian Dental Research Foundation grant 34-2013.