Journal article

constclust: Consistent Clusters for scRNA-seq

Isaac Virshup, Jarny Choi, Kim-Anh Lê Cao, Christine Wells

Cold Spring Harbor Laboratory | Published : 2020

Abstract

1 Unsupervised clustering to identify distinct cell types is a crucial step in the analysis of scRNA-seq data. Current clustering methods are dependent on a number of parameters whose effect on the resulting solution’s accuracy and reproducibility are poorly understood. The adjustment of clustering parameters is therefore ad-hoc, with most users deviating minimally from default settings. constclust is a novel meta-clustering method based on the idea that if the data contains distinct populations which a clustering method can identify, meaningful clusters should be robust to small changes in the parameters used to derive them. By reconciling solutions from a clustering method over multiple pa..

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