A comprehensive comparison of four methods for extracting lipids from Arabidopsis tissues
Cheka Kehelpannala, Thusitha WT Rupasinghe, Thomas Hennessy, David Bradley, Berit Ebert, Ute Roessner
Plant Methods | BioMed Central | Published : 2020
Background The plant lipidome is highly complex, and the composition of lipids in different tissues as well as their specific functions in plant development, growth and stress responses have yet to be fully elucidated. To do this, efficient lipid extraction protocols which deliver target compounds in solution at concentrations adequate for subsequent detection, quantitation and analysis through spectroscopic methods are required. To date, numerous methods are used to extract lipids from plant tissues. However, a comprehensive analysis of the efficiency and reproducibility of these methods to extract multiple lipid classes from diverse tissues of a plant has not been undertaken. Results In t..View full abstract
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Awarded by Australian Research Council Future Fellowship
Awarded by Mizutani Foundation for Glycoscience
CK is funded through a Research Training Program Scholarship provided by the Australian Commonwealth Government and the University of Melbourne. BE is supported by an Australian Research Council Future Fellowship [FT160100276] and a grant of the Mizutani Foundation for Glycoscience [18-0237].