Journal article

A comprehensive comparison of four methods for extracting lipids from Arabidopsis tissues

Cheka Kehelpannala, Thusitha WT Rupasinghe, Thomas Hennessy, David Bradley, Berit Ebert, Ute Roessner

Plant Methods | BioMed Central | Published : 2020

Abstract

Background The plant lipidome is highly complex, and the composition of lipids in different tissues as well as their specific functions in plant development, growth and stress responses have yet to be fully elucidated. To do this, efficient lipid extraction protocols which deliver target compounds in solution at concentrations adequate for subsequent detection, quantitation and analysis through spectroscopic methods are required. To date, numerous methods are used to extract lipids from plant tissues. However, a comprehensive analysis of the efficiency and reproducibility of these methods to extract multiple lipid classes from diverse tissues of a plant has not been undertaken. Results In t..

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Grants

Awarded by Australian Research Council Future Fellowship


Awarded by Mizutani Foundation for Glycoscience


Funding Acknowledgements

CK is funded through a Research Training Program Scholarship provided by the Australian Commonwealth Government and the University of Melbourne. BE is supported by an Australian Research Council Future Fellowship [FT160100276] and a grant of the Mizutani Foundation for Glycoscience [18-0237].