Journal article

Integrin alpha-2 and beta-1 expression increases through multiple generations of the EDW01 patient-derived xenograft model of breast cancer-insight into their role in epithelial mesenchymal transition in vivo gained from an in vitro model system

Razan Wafai, Elizabeth D Williams, Emma de Souza, Peter T Simpson, Amy E McCart Reed, Jamie R Kutasovic, Mark Waltham, Cameron E Snell, Tony Blick, Erik W Thompson, Honor J Hugo

Breast Cancer Research | BMC | Published : 2020

Abstract

BACKGROUND: Breast cancers acquire aggressive capabilities via epithelial to mesenchymal transition (EMT), in which various integrins/integrin-linked kinase signalling are upregulated. METHODS: We investigated this in two patient-derived xenografts (PDXs) developed from breast-to-bone metastases, and its functional significance in a breast cancer cell line system. ED03 and EDW01 PDXs were grown subcutaneously in immunocompromised SCID mice through 11 passages and 7 passages, respectively. Tumour tissue was assessed using immunohistochemistry (IHC) for oestrogen receptor (ER)-alpha, E-cadherin, vimentin, Twist1, beta-catenin, P120-RasGAP, CD44, CD24 and Ki67, and RT-qPCR of EMT-related factor..

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Grants

Awarded by National Health and Medical Research Council (Australia)


Awarded by National Breast Cancer Foundation, Australia (EMPathy National Collaborative Research Program)


Funding Acknowledgements

This research was supported in part by the National Health and Medical Research Council (Australia, #1027527) and the National Breast Cancer Foundation, Australia (EMPathy National Collaborative Research Program, CG-10-04).