Journal article

DNA methylation analysis of candidate genes associated with dementia in peripheral blood

Peter D Fransquet, Paul Lacaze, Richard Saffery, James Phung, Emily Parker, Raj C Shah, Anne Murray, Robyn L Woods, Joanne Ryan

Epigenomics | FUTURE MEDICINE LTD | Published : 2020

Abstract

Aim: To investigate whether genes implicated in dementia pathogenesis are differently methylated in peripheral blood. Materials & methods: Participants included 160 cognitively healthy individuals aged 70+ years: 73 who were subsequently diagnosed with dementia and 87 controls matched on age, gender, education, smoking and baseline cognition. A total of 49 participants also provided blood samples at diagnosis. Blood DNA methylation of APOE, APP, BDNF, PIN1, SNCA and TOMM40 was examined. Results: A total of 56 of 299 probes were differentially methylated in dementia compared with controls and 39 probes prior to diagnosis. The greatest effect size was in APP (cg19423170, Δ-8.32%, adjusted p = ..

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Grants

Awarded by National Cancer Institute at the National Institutes of Health


Awarded by National Health and Medical Research Council (NHMRC) of Australia


Awarded by NHMRC Dementia Research Leader Fellowship


Funding Acknowledgements

The work was supported by the National Institute on Aging and the National Cancer Institute at the National Institutes of Health (U01AG029824); the National Health and Medical Research Council (NHMRC) of Australia (334047 and 1127060); Monash University and the Victorian Cancer Agency. J Ryan is funded by an NHMRC Dementia Research Leader Fellowship (APP1135727). PD Fransquet is gratefully funded by RTP stipend PhD scholarship, awarded by Monash University and the Australian Government.