DNA methylation profiling identifies epigenetic differences between early versus late stages of diabetic chronic kidney disease.
Ashani Lecamwasam, Boris Novakovic, Braydon Meyer, Elif I Ekinci, Karen M Dwyer, Richard Saffery
Nephrol Dial Transplant | WILEY | Published : 2020
BACKGROUND: We investigated a cross-sectional epigenome-wide association study of patients with early and late diabetes-associated chronic kidney disease (CKD) to identify possible epigenetic differences between the two groups as well as changes in methylation across all stages of diabetic CKD. We also evaluated the potential of using a panel of identified 5'-C-phosphate-G-3' (CpG) sites from this cohort to predict the progression of diabetic CKD. METHODS: This cross-sectional study recruited 119 adults. DNA was extracted from blood using the Qiagen QIAampDNA Mini Spin Kit. Genome-wide methylation analysis was performed using Illumina Infinium MethylationEPIC BeadChips (HM850K). Intensity da..View full abstract