DNA methylation profiling identifies epigenetic differences between early versus late stages of diabetic chronic kidney disease

A Lecamwasam; B Novakovic; B Meyer; EI Ekinci; KM Dwyer; R Saffery

Journal article

DNA methylation profiling identifies epigenetic differences between early versus late stages of diabetic chronic kidney disease

A Lecamwasam, B Novakovic, B Meyer, EI Ekinci, KM Dwyer, R Saffery

Nephrology Dialysis Transplantation | OXFORD UNIV PRESS | Published : 2021

DOI: 10.1093/ndt/gfaa226

Abstract

Background: We investigated a cross-sectional epigenome-wide association study of patients with early and late diabetes-associated chronic kidney disease (CKD) to identify possible epigenetic differences between the two groups as well as changes in methylation across all stages of diabetic CKD. We also evaluated the potential of using a panel of identified 5′-C-phosphate-G-3′ (CpG) sites from this cohort to predict the progression of diabetic CKD. Methods: This cross-sectional study recruited 119 adults. DNA was extracted from blood using the Qiagen QIAampDNA Mini Spin Kit. Genome-wide methylation analysis was performed using Illumina Infinium MethylationEPIC BeadChips (HM850K). Intensity da..

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University of Melbourne Researchers

Boris Novakovic Author

Elif Ekinci Author

Karen Dwyer Author

Richard Saffery Author

Grants

Funding Acknowledgements

The authors declare an absence of any interests to disclose. There are no financial supports or associations that may pose a conflict of interest. A.L. is supported by a Deakin University School of Medicine Postgraduate Research Scholarship. B.N. is supported by a National Health and Medical Research Council (NHMRC) C.J. Martin Fellowship. E.I.E. is supported by the Sir Edward Weary Dunlop Medical Research Foundation Principal Research Fellowship at the University of Melbourne and the NHMRC. E.I.E.'s institution has received research funding from Novo Nordisk, Bayer, Sanofi and Dimerix.