Thymic iNKT single cell analyses unmask the common developmental program of mouse innate T cells.
S Harsha Krovi, Jingjing Zhang, Mary Jessamine Michaels-Foster, Tonya Brunetti, Liyen Loh, James Scott-Browne, Laurent Gapin
Nature Communications | Published : 2020
Most T lymphocytes leave the thymus as naïve cells with limited functionality. However, unique populations of innate-like T cells differentiate into functionally distinct effector subsets during their development in the thymus. Here, we profiled >10,000 differentiating thymic invariant natural killer T (iNKT) cells using single-cell RNA sequencing to produce a comprehensive transcriptional landscape that highlights their maturation, function, and fate decisions at homeostasis. Our results reveal transcriptional profiles that are broadly shared between iNKT and mucosal-associated invariant T (MAIT) cells, illustrating a common core developmental program. We further unmask a mutual requirement..View full abstract
Awarded by NCI NIH HHS