Journal article

Epigenetic differences at the HTR2A locus in progressive multiple sclerosis patients

Vicki E Maltby, Rodney A Lea, Sean Burnard, Alexandre Xavier, Thao Van Cao, Nicole White, Daniel Kennedy, Kira Groen, Katherine A Sanders, Rebecca Seeto, Samara Bray, Melissa Gresle, Louise Laverick, Helmut Butzkueven, Rodney J Scott, Jeannette Lechner-Scott

Scientific Reports | NATURE RESEARCH | Published : 2020

Abstract

The pathology of progressive multiple sclerosis (MS) is poorly understood. We have previously assessed DNA methylation in the CD4+ T cells of relapsing-remitting (RR) MS patients compared to healthy controls and identified differentially methylated regions (DMRs) in HLA-DRB1 and RNF39. This study aimed to investigate the DNA methylation profiles of the CD4+ T cells of progressive MS patients. DNA methylation was measured in two separate case/control cohorts using the Illumina 450K/EPIC arrays and data was analysed with the Chip Analysis Methylation Pipeline (ChAMP). Single nucleotide polymorphisms (SNPs) were assessed using the Illumina Human OmniExpress24 arrays and analysed using PLINK. Ex..

View full abstract

Grants

Awarded by Canadian Institutes of Health Research


Funding Acknowledgements

This study was supported by a grant from Multiple Sclerosis Research Australia (13-037, 14-027). RAL, VEM, RS and KAS are supported by fellowships from Multiple Sclerosis Research Australia. KAS was supported by a scholarship from the Trish foundation. VEM was supported by a fellowship from the Canadian Institutes of Health Research (320577).