Differences in pulmonary group 2 innate lymphoid cells are dependent on mouse age, sex and strain
Svenja Loering, Guy JM Cameron, Nirmal P Bhatt, Gabrielle T Belz, Paul S Foster, Philip M Hansbro, Malcolm R Starkey
Immunology and Cell Biology | WILEY | Published : 2021
Innate lymphoid cells (ILCs) are resident in the lung and are involved in both the maintenance of homeostasis and the pathogenesis of respiratory diseases. In this study, murine lung ILCs were characterized using flow cytometry and the impact of mouse age, sex and strain were assessed. Lung ILCs were found as early as postnatal day 4 and numbers peaked at 2 weeks, and then decreased as the lung matured. During postnatal lung development, ILC expressed differential amounts of group 2 ILC (ILC2)-associated cell surface antigens including ST2, CD90.2 and ICOS. Using Il5venus Il13td-tomato dual reporter mice, neonates were found to have increased constitutive interleukin (IL)-13 expression compa..View full abstract
Awarded by Australian Research Council
Awarded by National Health and Medical Research Council (NHMRC)
Awarded by NHMRC
MRS was supported by funding from Australian Research Council (DE170100226), National Health and Medical Research Council (NHMRC, APP1156898), Hunter Medical Research Institute, The University of Newcastle, Thoracic Society of Australian and New Zealand and Monash University. PMH is funded by Fellowships and grants from the NHMRC (1079187, 1175134) and by the University of Technology Sydney, University of Newcastle. GTB is funded by an NHMRC Fellowship (1135898). IL-5 reporter mice were kindly provided by Professor Paul Foster, The University of Newcastle, Australia. IL-13 reporter mice were kindly provided by Professor Andrew McKenzie, MRC Laboratory of Molecular Biology, Cambridge, United Kingdom.