Antifungal Activity and Molecular Mechanisms of Partial Purified Antifungal Proteins from Rhinacanthus nasutus against Talaromyces marneffei
Juthatip Jeenkeawpieam, Supachai Yodkeeree, Alex Andrianopoulos, Sittiruk Roytrakul, Monsicha Pongpom
JOURNAL OF FUNGI | MDPI | Published : 2020
Antifungal proteins (AFPs) are able to inhibit a wide spectrum of fungi without significant toxicity to the hosts. This study examined the antifungal activity of AFPs isolated from a Thai medicinal plant, Rhinacanthus nasutus, against the human pathogenic fungus Talaromycesmarneffei. This dimorphic fungus causes systemic infections in immunocompromised individuals and is endemic in Southeast Asian countries. The R. nasutus crude protein extract inhibited the growth of T. marneffei. The anti-T. marneffei activity was completely lost when treated with proteinase K and pepsin, indicating that the antifungal activity was dependent on a protein component. The total protein extract from R. nasutus..View full abstract
Awarded by National Research Council of Thailand
Awarded by Research Fund, Faculty of Medicine, Chiang Mai University
This research was funded and supported by National Research Council of Thailand: GSCMU(NRCT)/09/2562, Research Fund, Faculty of Medicine, Chiang Mai University: 092/2562, The Teaching Assistant and Research Assistant Scholarships, Graduate School, Chiang Mai University, and The Faculty of Medicine Graduate Student Scholarship, Chiang Mai University.