Journal article

Antifungal Activity and Molecular Mechanisms of Partial Purified Antifungal Proteins from Rhinacanthus nasutus against Talaromyces marneffei

Juthatip Jeenkeawpieam, Supachai Yodkeeree, Alex Andrianopoulos, Sittiruk Roytrakul, Monsicha Pongpom

JOURNAL OF FUNGI | MDPI | Published : 2020

Abstract

Antifungal proteins (AFPs) are able to inhibit a wide spectrum of fungi without significant toxicity to the hosts. This study examined the antifungal activity of AFPs isolated from a Thai medicinal plant, Rhinacanthus nasutus, against the human pathogenic fungus Talaromycesmarneffei. This dimorphic fungus causes systemic infections in immunocompromised individuals and is endemic in Southeast Asian countries. The R. nasutus crude protein extract inhibited the growth of T. marneffei. The anti-T. marneffei activity was completely lost when treated with proteinase K and pepsin, indicating that the antifungal activity was dependent on a protein component. The total protein extract from R. nasutus..

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University of Melbourne Researchers

Grants

Awarded by National Research Council of Thailand


Awarded by Research Fund, Faculty of Medicine, Chiang Mai University


Funding Acknowledgements

This research was funded and supported by National Research Council of Thailand: GSCMU(NRCT)/09/2562, Research Fund, Faculty of Medicine, Chiang Mai University: 092/2562, The Teaching Assistant and Research Assistant Scholarships, Graduate School, Chiang Mai University, and The Faculty of Medicine Graduate Student Scholarship, Chiang Mai University.