Effect of Rice GDP-L-Galactose Phosphorylase Constitutive Overexpression on Ascorbate Concentration, Stress Tolerance, and Iron Bioavailability in Rice
Ronan C Broad, Julien P Bonneau, Jesse T Beasley, Sally Roden, Pawel Sadowski, Nathaniel Jewell, Chris Brien, Bettina Berger, Elad Tako, Raymond P Glahn, Roger P Hellens, Alexander AT Johnson
Frontiers in Plant Science | FRONTIERS MEDIA SA | Published : 2020
Ascorbate (vitamin C) is an essential multifunctional molecule for both plants and mammals. In plants, ascorbate is the most abundant water-soluble antioxidant that supports stress tolerance. In humans, ascorbate is an essential micronutrient and promotes iron (Fe) absorption in the gut. Engineering crops with increased ascorbate levels have the potential to improve both crop stress tolerance and human health. Here, rice (Oryza sativa L.) plants were engineered to constitutively overexpress the rice GDP-L-galactose phosphorylase coding sequence (35S-OsGGP), which encodes the rate-limiting enzymatic step of the L-galactose pathway. Ascorbate concentrations were negligible in both null segrega..View full abstract
This research was supported by an Australian Plant Phenomics Facility Postgraduate Student Internship Award to RB and grants from the United States Department of Agriculture, Agricultural Research Service to RG.