Journal article

Low levels of Grainy head isoforms regulate Drosophila midgut intestinal stem cell differentiation

Nicole Dominado, Franca Casagranda, Nicole Siddall, Helen Abud, Gary Hime

Cold Spring Harbor Laboratory | Published : 2020


Regeneration of the Drosophila midgut epithelium depends upon differential expression of transcription factors in intestinal stem cells and their progeny. The grainy head locus produces multiple splice forms that result in production of two classes of transcription factor, designated Grh.O and Grh.N. grainy head is expressed at very low levels in the midgut and yet Grh.O is required for maintenance of intestinal stem cells and exhibits a second function regulating enteroblast differentiation in conjunction with miR-8 and Zfh-1. Grh.O expression must be tightly regulated as high level ectopic expression in enteroblasts results in cells with confused identity and promotes excess proliferation ..

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