Journal article

A modified assay for the enumeration of ascaris eggs in fresh raw sewage

Esmaeil Shahsavari, Jonathan Schmidt, Arturo Aburto-Medina, Basma Khallaf, Vivek Balakrishnan, Nicholas D Crosbie, Aravind Surapaneni, Andrew S Ball

MethodsX | ELSEVIER SCIENCE BV | Published : 2017


Soil-transmitted helminths (STHs) pose a significant public health problem, infecting approximately 2 billion people globally. Despite relatively low prevalence in developed countries, the removal of STHs from wastewater remains crucial to allow the safe use of biosolids or recycled water for agriculture. Wastewater helminth egg count data can contribute to an assessment of the need for, or success of, a parasite management program. Although the World Health Organisation (WHO) has recommended a standard method for counting helminth eggs in raw sewage based on the method of Bailenger (Ayres et al., 1996), the method generally results in low percentage egg recoveries. Given the importance of d..

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Funding Acknowledgements

This study was funded by South East Water and Melbourne Water. Thank you to Dr. Greg Sturbaum, Dr. Chi-Wen Tseng, Connie Lee and Kate Broadhurst at ALS Water for optimising the method and processing the samples. MethodsX thanks the reviewers of this article for taking the time to provide valuable feedback.