Journal article

Emerging recombinant noroviruses identified by clinical and waste water screening

Jennifer H Lun, Joanne Hewitt, Alefiya Sitabkhan, John-Sebastian Eden, Daniel Enosi Tuipulotu, Natalie E Netzler, Leigh Morrell, Juan Merif, Richard Jones, Bixing Huang, David Warrilow, Kelly-Anne Ressler, Mark J Ferson, Dominic E Dwyer, Jen Kok, William D Rawlinson, Daniel Deere, Nicholas D Crosbie, Peter A White



Norovirus is estimated to cause 677 million annual cases of gastroenteritis worldwide, resulting in 210,000 deaths. As viral gastroenteritis is generally self-limiting, clinical samples for epidemiological studies only partially represent circulating noroviruses in the population and is biased towards severe symptomatic cases. As infected individuals from both symptomatic and asymptomatic cases shed viruses into the sewerage system at a high concentration, waste water samples are useful for the molecular epidemiological analysis of norovirus genotypes at a population level. Using Illumina MiSeq and Sanger sequencing, we surveyed circulating norovirus within Australia and New Zealand, from Ju..

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Awarded by National Health and Medical Research Council

Awarded by NHMRC

Funding Acknowledgements

This Australian research was supported by funding from the National Health and Medical Research Council (project number APP1083139 and APP1123135). The New Zealand research was funded by the Ministry of Health, New Zealand. We thank Dawn Croucher from the Kenepuru Science Centre for her technical input. We would like to thank our colleagues Alper Yasar and Jason Koval from the Ramaciotti Centre for Genomics (UNSW) for advice and technical support with MiSeq sequencing. We also thank Neil Franklin and Keira Glasgow (NSW Ministry of Health) for institutional outbreak data, and Melbourne Water Corporation and Sydney Water Corporation for provision of waste water samples. JHL, DET and NEN were supported by an Australian Postgraduate Award, and JHL was additionally supported by a Water Research Australia Scholarship. J-SE is supported by an NHMRC Early Career Fellowship (1073466).