Journal article

A quantitative test for heat-induced cell necrosis in vascular cambium and secondary phloem of Eucalyptus obliqua stems

Yasika Medhavi Subasinghe Achchige, Liubov Volkova, Andrew Drinnan, Christopher J Weston

Journal of Plant Ecology | Oxford University Press (OUP) | Published : 2020


Aims Exposure of Eucalyptus tree stems to the radiant heat of forest fires can kill cambial cells and their embedded regenerative meristems, thus preventing epicormic resprouting and recovery of the tree. Currently there is no tissue-level method to quantify the viability of cambial cells in Eucalyptus following heat exposure. The first aim of this study was to adapt and validate the tetrazolium reduction method of testing for cell viability in Eucalyptus. The second aim was to apply the method to establish a threshold level of cambium cell viability in Eucalyptus obliqua to enable the identification of a critical temperature. Methods The study used the tetrazolium reduction method to quant..

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Funding Acknowledgements

The authors acknowledge Dr. Lisa Wittick for sharing the spectrophotometer, Rajitha Ratnayake for field and technical assistance during project implementation. Yasika Subasinghe Achchige acknowledges the Melbourne Research Scholarship from The University of Melbourne.