Journal article

The focal adhesion targeting domain of p130Cas confers a mechanosensing function

Peta M Bradbury, Kylie Turner, Camilla Mitchell, Kaitlyn R Griffin, Shiloh Middlemiss, Loretta Lau, Rebecca Dagg, Elena Taran, Justin Cooper-White, Ben Fabry, Geraldine M O'Neill

JOURNAL OF CELL SCIENCE | COMPANY OF BIOLOGISTS LTD | Published : 2017

Abstract

Members of the Cas family of focal adhesion proteins contain a highly conserved C-terminal focal adhesion targeting (FAT) domain. To determine the role of the FAT domain in these proteins, we compared wild-type exogenous NEDD9 with a hybrid construct in which the NEDD9 FAT domain had been exchanged for the p130Cas (also known as BCAR1) FAT domain. Fluorescence recovery after photobleaching (FRAP) revealed significantly slowed exchange of the fusion protein at focal adhesions and significantly slower two-dimensional migration. No differences were detected in cell stiffness as measured using atomic force microscopy (AFM) and in cell adhesion forces measured with a magnetic tweezer device. Thus..

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Grants

Awarded by New South Wales Cancer Council (Cancer Council NSW)


Funding Acknowledgements

This work was supported by the New South Wales Cancer Council (Cancer Council NSW) (RG12/06 to G.M.O. and B.F.), a University of Sydney Strategic Priority Areas for Collaboration Grant and generous funding through Dooley's Lidcombe Catholic Club. P.M.B. holds a University of Sydney postgraduate student scholarship and received a German Academic Exchange Service award (Deutscher Akademischer Austauschdienst). C.M. was supported by a C4-fellowship from the Kids Cancer Project.