Journal article
Size-dependent gene delivery of amine-modified silica nanoparticles
Meihua Yu, Yuting Niu, Jun Zhang, Hongwei Zhang, Yannan Yang, Elena Taran, Siddharth Jambhrunkar, Wenyi Gu, Peter Thorn, Chengzhong Yu
NANO RESEARCH | TSINGHUA UNIV PRESS | Published : 2016
Abstract
Silica-based nanoparticles are promising carriers for gene delivery applications. To gain insights into the effect of particle size on gene transfection efficiency, amine-modified monodisperse Stöber spheres (NH2-SS) with diameters of 125, 230, 330, 440, and 570 nm were synthesized. The in vitro transfection efficiencies of NH2-SS for delivering plasmid DNA encoding green fluorescent protein (GFP) (pcDNA3-EGFP, abbreviated as pcDNA, 6.1 kbp) were studied in HEK293T cells. NH2-SS with a diameter of 330 nm (NH2-SS330) showed the highest GFP transfection level compared to NH2-SS particles with other sizes. The transfection efficiency was found as a compromise between the binding capacity and ce..
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Funding Acknowledgements
We thank the support from Australian Research Council, the Australian Microscopy & Microanalysis Research Facility at the Centre for Microscopy and Microanalysis, the University of Queensland, and the Queensland node of the Australian National Fabrication Facility (ANFF).