Synthesis of silica nanoparticles with controllable surface roughness for therapeutic protein delivery
Yuting Niu, Meihua Yu, Jun Zhang, Yannan Yang, Chun Xu, Michael Yeh, Elena Taran, Jeff Jia Cheng Hou, Peter P Gray, Chengzhong Yu
Journal of Materials Chemistry B | ROYAL SOC CHEMISTRY | Published : 2015
There is an increasing demand of efficient nano-carriers for intracellular delivery of therapeutic proteins. This study reports on a novel "neck-enhancing" approach to synthesize stable rough silica nanoparticles (RSNs) with controllable surface roughness. By increasing the shell particle size from 13 to 98 nm while fixing the core size at 211 nm, the interspace size between neighboring shell particles of RSNs is enlarged from 7 to 38 nm. Cytochrome c, IgG fragment and IgG antibody are preferably adsorbed onto one of the RSNs with the interspace size of 14, 21 and 38 nm, respectively. The binding activity of the IgG fragment loaded onto RSNs is maintained as confirmed by surface plasmon reso..View full abstract
The authors acknowledge the Australian Research Council for financial support. We are grateful for use of the facilities, and scientific and technical assistance, of the Australian National Fabrication Facility and the Australian Microscopy and Microanalysis Research Facility at the Centre for Microscopy and Microanalysis, The University of Queensland.