Escherichia coli strains expressing H12 antigens demonstrate an increased ability to attach to abiotic surfaces as compared with E-coli strains expressing H7 antigens
Rebecca M Goulter, Elena Taran, Ian R Gentle, Kari S Gobius, Gary A Dykes
Colloids and Surfaces B Biointerfaces | ELSEVIER SCIENCE BV | Published : 2014
The role of Escherichia coli H antigens in hydrophobicity and attachment to glass, Teflon and stainless steel (SS) surfaces was investigated through construction of fliC knockout mutants in E. coli O157:H7, O1:H7 and O157:H12. Loss of FliC(H12) in E. coli O157:H12 decreased attachment to glass, Teflon and stainless steel surfaces (p0.05), but complementation with cloned fliC(H12), as opposed to cloned fliCH7, significantly increased attachment for both strains compared with wt counterparts (p<0.05). Hydrophobicity determined using bacterial adherence to hydrocarbons and contact angle measurements differed with fliC expression but was not correlated to the attachment to materials included in ..View full abstract
R.M. Coulter acknowledges the financial support of the Department of Employment, Economic Development and Innovation of the Queensland Government, Australia through the Smart State PhD Scholarships Program and the financial support of the Australian Government through the Australian Postgraduate Award. This work was also supported through funding from CSIRO Food and Nutritional Sciences.