Comparison and evaluation of immobilization methods for preparing bacterial probes using acidophilic bioleaching bacteria Acidithiobacillus thiooxidans for AFM studies
Mengxue Diao, Elena Taran, Stephen M Mahler, Anh V Nguyen
Journal of Microbiological Methods | ELSEVIER SCIENCE BV | Published : 2014
We evaluated different strategies for constructing bacterial probes for atomic force microscopy studies of bioleaching Acidithiobacillus thiooxidans interacting with pyrite mineral surfaces. Of three available techniques, the bacterial colloidal probe technique is the most reliable and provides a versatile platform for quantifying true interactive forces between bioleaching microorganisms and mineral surfaces.
The authors would like to acknowledge the University of Queensland for a UQRS scholarship (MD) and the CMM facilities, and Dr. Tuan Nguyen's help with SEM.