CsgA Production by Escherichia coli O157:H7 Alters Attachment to Abiotic Surfaces in Some Growth Environments
RM Goulter-Thorsen, E Taran, IR Gentle, KS Gobius, GA Dykes
Applied and Environmental Microbiology | AMER SOC MICROBIOLOGY | Published : 2011
The role of curli expression in attachment of Escherichia coli O157:H7 to glass, Teflon, and stainless steel (SS) was investigated through the creation of csgA knockout mutants in two isolates of E. coli O157:H7. Attachment assays using epifluorescence microscopy and measurements of the force of adhesion of bacterial cells to the substrates using atomic force microscopy (AFM) force mapping were used to determine differences in attachment between wild-type (wt) and csgA-negative (ΔcsgA) strains following growth in four different media. The hydrophobicity of the cells was determined using contact angle measurements (CAM) and bacterial adhesion to hydrocarbons (BATH). The attachment assay resul..View full abstract
R. M. Goulter-Thorsen acknowledges the financial support of the Department of Employment, Economic Development and Innovation of the Queensland Government, Australia, through the Smart State Ph.D. Scholarships Program and the financial support of the Australian Government through an Australian Postgraduate Award. This work was supported through funding by CSIRO Food and Nutritional Sciences.