Rapid One-Step Selection Method for Generating Nucleic Acid Aptamers: Development of a DNA Aptamer against alpha-Bungarotoxin
Lasse H Lauridsen, Hadi A Shamaileh, Stacey L Edwards, Elena Taran, Rakesh N Veedu
PLoS One | PUBLIC LIBRARY SCIENCE | Published : 2012
BACKGROUND: Nucleic acids based therapeutic approaches have gained significant interest in recent years towards the development of therapeutics against many diseases. Recently, research on aptamers led to the marketing of Macugen®, an inhibitor of vascular endothelial growth factor (VEGF) for the treatment of age related macular degeneration (AMD). Aptamer technology may prove useful as a therapeutic alternative against an array of human maladies. Considering the increased interest in aptamer technology globally that rival antibody mediated therapeutic approaches, a simplified selection, possibly in one-step, technique is required for developing aptamers in limited time period. PRINCIPAL FIN..View full abstract
Awarded by University of Queensland ECR grant
Awarded by Novo Nordisk Fonden
The work was supported by The University of Queensland ECR grant (Project ID 2010002143) awarded to RNV. LHL acknowledges the Novo Nordisk Foundation for supporting his stay at The University of Queensland, Australia. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.