Journal article

Human arterial smooth muscle cells synthesize granulocyte colony- stimulating factor in response to interleukin-1 alpha and tumor necrosis factor-alpha

H Zoellner, EL Filonzi, HR Stanton, JE Layton, JA Hamilton

Blood | American Society of Hematology | Published : 1992


Vascular smooth muscle cells (SMC) are a major cell type comprising the walls of blood vessels. We report the synthesis of granulocyte colony- stimulating factor (G-CSF) by cultured human SMC obtained from the internal mammary artery and thoracic aorta. Interleukin-1 alpha (IL-1 alpha) greatly increased in a dose-dependent manner the amount of this cytokine produced by the SMC, with tumor necrosis factor-alpha (TNF- alpha) being less effective. Newly formed G-CSF could be detected in culture supernatants within 6 hours after IL-1 alpha or TNF-alpha treatment. Northern blot analysis of SMC stimulated with IL-1 alpha and TNF-alpha showed an increase in the amount of mRNA for G-CSF as compared ..

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