Journal article

The Contribution of lincRNAs at the Interface between Cell Cycle Regulation and Cell State Maintenance

Adriano Biasini, Adam Alexander Thil Smith, Baroj Abdulkarim, Maria Ferreira da Silva, Jennifer Yihong Tan, Ana Claudia Marques

iScience | CELL PRESS | Published : 2020

Abstract

Cell cycle progression is controlled by the interplay of established cell cycle regulators. Changes in these regulators' activity underpin differences in cell cycle kinetics between cell types. We investigated whether long intergenic noncoding RNAs (lincRNAs) contribute to embryonic stem cell cycle adaptations. Using single-cell RNA sequencing data for mouse embryonic stem cells (mESCs) staged as G1, S, or G2/M we found differentially expressed lincRNAs are enriched among cell cycle-regulated genes. These lincRNAs (CC-lincRNAs) are co-expressed with genes involved in cell cycle regulation. We tested the impact of two CC-lincRNA candidates and show using CRISPR activation that increasing thei..

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Grants

Awarded by Swiss National Science Foundation


Funding Acknowledgements

We would like to thank the University of Lausanne Flow Cytometry Platformfor help with FACS and sorting analysis. The computations were performed at the Vital-IT (https://www.vital-it.ch) Center for high-performance computing of the SIB Swiss Institute of Bioinformatics. We would like to thank Dario Bottinelli for technical support during the early stages of this project and Vincent Dion and Constance Ciaudo for reading and commenting on early versions of this manuscript. This work was funded by the Swiss National Science Foundation (grant PP00P3_150667 to A. C. M.) and the NCCR in RNA & Disease (A.C.M.).