N-glycosylation and expression in human tissues of the orphan GPR61 receptor.
Paweł Kozielewicz, Hatun Alomar, Syaratul Yusof, Gillian Grafton, Alison J Cooper, S John Curnow, James W Ironside, Hardev Pall, Nicholas M Barnes
FEBS Open Bio | Published : 2017
A number of members of the G protein-coupled receptor class of cell surface receptors are 'orphans' with no known endogenous ligand. One of these orphan receptors is GPR61; there are little data about its expression in human cells and tissues. In this study, we investigated the post-translational modification of GPR61 by N-glycosylation at an identified consensus N-glycosylation site (N12) and the impact of this modification upon the subcellular expression of the protein. The N-glycosylation inhibitor tunicamycin reduced the apparent molecular weight of immunoreactivity associated with myc-tagged GPR61 by 1-2 kDa, which was comparable to the evident molecular weight of the myc-tagged N12S GP..View full abstract