Journal article

The App(NL-G-F) mouse retina is a site for preclinical Alzheimer's disease diagnosis and research

Marjan Vandenabeele, Lien Veys, Sophie Lemmens, Xavier Hadoux, Geraldine Gelders, Luca Masin, Lutgarde Serneels, Jan Theunis, Takashi Saito, Takaomi C Saido, Murali Jayapala, Patrick De Boever, Bart De Strooper, Ingeborg Stalmans, Peter van Wijngaarden, Lieve Moons, Lies De Groef

Acta Neuropathologica Communications | BMC | Published : 2021

Abstract

In this study, we report the results of a comprehensive phenotyping of the retina of the AppNL-G-F mouse. We demonstrate that soluble Aβ accumulation is present in the retina of these mice early in life and progresses to Aβ plaque formation by midlife. This rising Aβ burden coincides with local microglia reactivity, astrogliosis, and abnormalities in retinal vein morphology. Electrophysiological recordings revealed signs of neuronal dysfunction yet no overt neurodegeneration was observed and visual performance outcomes were unaffected in the AppNL-G-F mouse. Furthermore, we show that hyperspectral imaging can be used to quantify retinal Aβ, underscoring its potential as a biomarker for AD di..

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Grants

Funding Acknowledgements

MV, LV, LuM and LDG are fellows of the Research Foundation Flanders (FWO). This research was supported by the Alzheimer's Research Foundation (SAO-FRA), the European Union Horizon 2020 Research and Innovation Program (2014-2020) (HERALD project, granted by the ATTRAC T consortium). The Centre for Eye Research Australia receives Operational Infrastructure Support from the Victorian Government. XH and PvW acknowledge funding support from the H & L Hecht Trust and the Yulgilbar Alzheimer's Research Program.