Intestinal-derived ILCs migrating in lymph increase IFN gamma production in response to Salmonella Typhimurium infection
Verena Kastele, Johannes Mayer, Edward S Lee, Natalie Papazian, John J Cole, Vuk Cerovic, Gabrielle Belz, Michio Tomura, Gerard Eberl, Carl Goodyear, Rose A Maciewicz, Daniel Wall, Tom Cupedo, David R Withers, Simon Milling
Mucosal Immunology | SPRINGERNATURE | Published : 2021
Innate lymphoid cells (ILCs) are enriched in mucosae and have been described as tissue-resident. Interestingly, ILCs are also present within lymph nodes (LNs), in the interfollicular regions, the destination for lymph-migratory cells. We have previously shown that LN ILCs are supplemented by peripheral tissue-derived ILCs. Using thoracic duct cannulations, we here enumerate the intestinal lymph ILCs that traffic from the intestine to the mesenteric LNs (MLNs). We provide, for the first time, a detailed characterisation of these lymph-migratory ILCs. We show that all ILC subsets migrate in lymph, and while global transcriptional analysis reveals a shared signature with tissue-resident ILCs, l..View full abstract
Awarded by Versus Arthritis Rheumatoid Arthritis Pathogenesis Centre for Excellence (RACE)
Awarded by Medical Research Council
We thank Diane Vaughan and the Flow Cytometry Core Facility for expert assistance and Dr. Matthew Hepworth for critical review of our manuscript. V.K. was supported by the Versus Arthritis Rheumatoid Arthritis Pathogenesis Centre for Excellence (RACE) (grant number 20298). J.M. was supported by the Wellcome Trust "Molecular Functions in Disease" Doctoral Training Programme. J.J.C. was supported by the GLAZgo Discovery Centre. V.C. was supported by a project grant from the Medical Research Council (MR/K021095/1).