Journal article
Plasticity of distal nephron epithelia from human kidney organoids enables the induction of ureteric tip and stalk
SE Howden, SB Wilson, E Groenewegen, L Starks, TA Forbes, KS Tan, JM Vanslambrouck, EM Holloway, YH Chen, S Jain, JR Spence, MH Little
Cell Stem Cell | CELL PRESS | Published : 2021
Abstract
Little and colleagues demonstrate the plasticity of the distal nephron epithelium present within kidney organoids, showing that this can be induced to adopt a ureteric epithelial phenotype. Subsequent maturation of this epithelium generated collecting ducts, facilitating the accurate modeling of autosomal recessive polycystic kidney disease.
Grants
Awarded by National Institute of Diabetes and Digestive and Kidney Diseases
Funding Acknowledgements
We thank the Australian Genome Research Facility for access to 10x Chromium single cell library preparation and the Murdoch Children's Research Institute Translational Genomics Unit for provision of Next Generation Sequencing. M.H.L. is a Senior Principal Research Fellow of the National Health and Medical Research Council, Australia (APP1136085). This work was supported by the National Institutes of Health (UH3DK107344; U01DK107350), Australian Research Council (DP190101705), and the Dutch Kidney Foundation (RECORD KID). The primary kidney scRNA-seq was supported by grant number CZF2019-002440 from the Chan Zuckerberg Initiative DAF, an advised fund of Silicon Valley Community Foundation awarded to J.R.S. We thank the Alvin J. Siteman Cancer Center at Washington University School of Medicine and Barnes-Jewish Hospital in St. Louis, MO for the use of the Genome Engineering and Induced Pluripotent Stem Cell Center, which provided gene editing service for the Wnt9B iPSC reporter line, and Bendi Gong for technical assistance with this line. The Siteman Cancer Center is supported in part by an NCI Cancer Center Support Grant #P30 CA09184.