IgE and IgG(4) epitopes revealed on the major fish allergen Lat c 1
Michael F Sharp, Aya C Taki, Thimo Ruethers, Juan N Stephen, Norelle L Daly, Andreas L Lopata, Sandip D Kamath
MOLECULAR IMMUNOLOGY | PERGAMON-ELSEVIER SCIENCE LTD | Published : 2021
BACKGROUND: The IgE- and IgG4-binding patterns of the major fish allergen parvalbumins are not clearly understood. IgE antibody-binding to parvalbumin from Asian seabass, Lat c 1.01, is implicated in up to 90 % of allergic reactions, although the region of IgE or IgG4 epitopes are unknown. In the present study, we characterized the specific IgE- and IgG4-binding regions of Lat c 1.01 using serum from pediatric and adult patients with clinically-confirmed fish allergy. METHODS: A comparative investigation of patient IgE- and IgG4-binding to recombinant Lat c 1.01 was performed by immunoblotting and indirect ELISA using serum from 15 children and eight adults with clinically confirmed IgE-medi..View full abstract
Awarded by National Health and Medical Research Council (NHMRC)
The study was supported by an Australian Research Council fellowship and a National Health and Medical Research Council (NHMRC) grant to A.L.L. (APP1086656). T.R. is a full-time PhD scholar of the Centre for Food and Allergy Research and James Cook University, Australia. S.D.K. is an National Health and Medical Research Council (NHMRC) Peter Doherty Early Career Research fellow (GNT1124143).