Journal article

Multi-level remodelling of chromatin underlying activation of human T cells

Naiara G Bediaga, Hannah D Coughlan, Timothy M Johanson, Alexandra L Garnham, Gaetano Naselli, Jan Schroeder, Liam G Fearnley, Esther Bandala-Sanchez, Rhys S Allan, Gordon K Smyth, Leonard C Harrison



Remodelling of chromatin architecture is known to regulate gene expression and has been well characterized in cell lineage development but less so in response to cell perturbation. Activation of T cells, which triggers extensive changes in transcriptional programs, serves as an instructive model to elucidate how changes in chromatin architecture orchestrate gene expression in response to cell perturbation. To characterize coordinate changes at different levels of chromatin architecture, we analyzed chromatin accessibility, chromosome conformation and gene expression in activated human T cells. T cell activation was characterized by widespread changes in chromatin accessibility and interactio..

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Awarded by Australian National Health and Medical Research Council (NHMRC)

Awarded by Centre for Research Excellence Grant

Funding Acknowledgements

We thank Dr. Stephen Wilcox (Genomics Hub, WEHI) for generating the sequencing data and Drs. Natasha Jansz and Marnie Blewitt for their advice on the ATAC-seq protocol. This work was supported by Australian National Health and Medical Research Council (NHMRC) Program Grant (APP1037321), Research Fellowships (H.D.C, T.M.J, L.F, G.K.S, R.S.A, L.C.H) and Centre for Research Excellence Grant (APP 1078106) (N.G.B.). The work was made possible through Victorian State Government Operational Infrastructure Support and NHMRC Research Institute Infrastructure Support Scheme.