Journal article

Insulin regulates human mammosphere development and function

Ashalyn P Watt, Christophe Lefevre, Cynthia S Wong, Kevin R Nicholas, Julie A Sharp

CELL AND TISSUE RESEARCH | SPRINGER | Published : 2021

Abstract

Assessing the role of lactogenic hormones in human mammary gland development is limited due to issues accessing tissue samples and so development of a human in vitro three-dimensional mammosphere model with functions similar to secretory alveoli in the mammary gland can aid to overcome this shortfall. In this study, a mammosphere model has been characterised using human mammary epithelial cells grown on either mouse extracellular matrix or agarose and showed insulin is essential for formation of mammospheres. Insulin was shown to up-regulate extracellular matrix genes. Microarray analysis of these mammospheres revealed an up-regulation of differentiation, cell-cell junctions, and cytoskeleto..

View full abstract