Quantitative analysis of the splice variants expressed by the major hepatitis B virus genotypes
Chun Shen Lim, Vitina Sozzi, Margaret Littlejohn, Lilly KW Yuen, Nadia Warner, Brigid Betz-Stablein, Fabio Luciani, Peter A Revill, Chris M Brown
MICROBIAL GENOMICS | MICROBIOLOGY SOC | Published : 2021
Hepatitis B virus (HBV) is a major human pathogen that causes liver diseases. The main HBV RNAs are unspliced transcripts that encode the key viral proteins. Recent studies have shown that some of the HBV spliced transcript isoforms are predictive of liver cancer, yet the roles of these spliced transcripts remain elusive. Furthermore, there are nine major HBV genotypes common in different regions of the world, these genotypes may express different spliced transcript isoforms. To systematically study the HBV splice variants, we transfected human hepatoma cells, Huh7, with four HBV genotypes (A2, B2, C2 and D3), followed by deep RNA-sequencing. We found that 13-28 % of HBV RNAs were splice var..View full abstract
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Awarded by Marjorie McCallum travel award - National Health and Medical Research Council (NHMRC)
C. M. B. and C. S. L. were funded by the University of Otago. C. S. L. was a recipient of a Dr Sulaiman Daud 125th Jubilee Postgraduate Scholarship and the Marjorie McCallum travel award. P. A. R., V. S. and M. L. were funded by National Health and Medical Research Council (NHMRC) grant APP1145977.