Journal article

Quantitative analysis of the splice variants expressed by the major hepatitis B virus genotypes

Chun Shen Lim, Vitina Sozzi, Margaret Littlejohn, Lilly KW Yuen, Nadia Warner, Brigid Betz-Stablein, Fabio Luciani, Peter A Revill, Chris M Brown



Hepatitis B virus (HBV) is a major human pathogen that causes liver diseases. The main HBV RNAs are unspliced transcripts that encode the key viral proteins. Recent studies have shown that some of the HBV spliced transcript isoforms are predictive of liver cancer, yet the roles of these spliced transcripts remain elusive. Furthermore, there are nine major HBV genotypes common in different regions of the world, these genotypes may express different spliced transcript isoforms. To systematically study the HBV splice variants, we transfected human hepatoma cells, Huh7, with four HBV genotypes (A2, B2, C2 and D3), followed by deep RNA-sequencing. We found that 13-28 % of HBV RNAs were splice var..

View full abstract

University of Melbourne Researchers