Journal article

Fates of Antibiotic Resistance Genes in the Gut Microbiome from Different Soil Fauna under Long-Term Fertilization

Fei Zheng, Qing-Fang Bi, Madeline Giles, Roy Neilson, Qing-Lin Chen, Xian-Yong Lin, Yong-Guan Zhu, Xiao-Ru Yang

ENVIRONMENTAL SCIENCE & TECHNOLOGY | AMER CHEMICAL SOC | Published : 2021

Abstract

Applying organic fertilizers has been well documented to facilitate the dissemination of antibiotic resistance genes (ARGs) in soil ecosystems. However, the role of soil fauna in this process has been seldom addressed, which hampers our ability to predict the fate of and to manage the spread of ARGs. Here, using high-throughput quantitative polymerase chain reaction (HT-qPCR), we examined the effect of long-term (5-, 8-, and 10-year) fertilization treatments (control, inorganic fertilizers, and mixed fertilizers) on the transfer of ARGs between soil, nematodes, and earthworms. We found distinct fates for ARGs in the nematodes and earthworms, with the former having higher enriched levels of A..

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University of Melbourne Researchers

Grants

Awarded by National Key Research and Development Program of China-International collaborative project from the Ministry of Science and Technology


Awarded by National Natural Science Foundation of China


Funding Acknowledgements

This work was supported by the National Key Research and Development Program of China-International collaborative project from the Ministry of Science and Technology (2017YFE0107300), the National Natural Science Foundation of China (41977201, 41571130063), and the K.C. Wong Education Foundation. We appreciated Bingjie Jin for collection and pretreatment of a part of samples and Shuyidan Zhou for technical assistance. The James Hutton Institute receives financial support from the Scottish Government Rural and Environment Science and Analytical Services (RESAS).