Journal article

Expression of EGFR and conformational forms of EGFR in malignant pleural mesothelioma and its impact on survival

Puey Ling Chia, Sagun Parakh, Prudence Russell, Hui K Gan, Khashayer Asadi, Val Gebski, Carmel Murone, Marzena Walkiewicz, Zhanqi Liu, Bibhusal Thapa, Fiona E Scott, Andrew M Scott, Thomas John

LUNG CANCER | ELSEVIER IRELAND LTD | Published : 2021

Abstract

AIM: Conformational forms of the epidermal growth factor receptor (EGFR) are pro-tumorigenic. The prevalence and impact of conformational forms of EGFR in malignant mesothelioma (MM) is unknown. We investigated expression of EGFR and conformational forms of EGFR by immunohistochemistry using EGFR-targeting monoclonal antibodies (mAb). In addition, EGFR gene amplification was investigated by fluorescent in-situ hybridization (FISH). Findings were correlated with survival. METHODS: Patients treated between 1988 and 2014 were identified from the thoracic surgery database of the Austin Hospital, Melbourne, Australia. Tissue microarrays (TMAs) were constructed, subjected to wild type (wt) EGFR IH..

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Grants

Awarded by National Health and Medical Research Council (NHMRC)


Funding Acknowledgements

This study was supported by the Mesothelioma grant awarded by Cancer Council Victoria and Victorian Cancer Agency (TJ), IASLC Lung Cancer Fellowship (PLC), University of Melbourne Australian Postgraduate Award (PLC), the National Health and Medical Research Council (NHMRC) fellowship 1084178 (AMS) and program grant 1092788. This work was supported, in part, by funds from the Operational Infrastructure Support Program provided by the Victorian Government, Australia. We also acknowledge Dr Peter Lock, Bioimaging facility, La Trobe University, for his assistance with EGFR FISH analysis.