Journal article

Association of variably methylated tumour DNA regions with overall survival for invasive lobular breast cancer

Medha Suman, Pierre-Antoine Dugue, Ee Ming Wong, JiHoon Eric Joo, John L Hopper, Tu Nguyen-Dumont, Graham G Giles, Roger L Milne, Catriona McLean, Melissa C Southey



BACKGROUND: Tumour DNA methylation profiling has shown potential to refine disease subtyping and improve the diagnosis and prognosis prediction of breast cancer. However, limited data exist regarding invasive lobular breast cancer (ILBC). Here, we investigated the genome-wide variability of DNA methylation levels across ILBC tumours and assessed the association between methylation levels at the variably methylated regions and overall survival in women with ILBC. METHODS: Tumour-enriched DNA was prepared by macrodissecting formalin-fixed paraffin embedded (FFPE) tumour tissue from 130 ILBCs diagnosed in the participants of the Melbourne Collaborative Cohort Study (MCCS). Genome-wide tumour DN..

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Awarded by National Breast Cancer Foundation (Australia)

Awarded by National Health and Medical Research Council (NMHRC, Australia)

Awarded by NHMRC

Awarded by Cancer Australia

Awarded by Australian National Health and Medical Research Council

Funding Acknowledgements

MS was the recipient of a Beaney Scholarship in Pathology from The University of Melbourne (2018). TN-D a National Breast Cancer Foundation (Australia) Career Development Fellow (ECF-17-001). MCS is a National Health and Medical Research Council (NMHRC, Australia) Senior Research Fellow (APP1155163). This work was supported by an NHMRC Program Grant (APP1074383) and a project grant from Cancer Australia (APP1047347). Melbourne Collaborative Cohort Study (MCCS) cohort recruitment was funded by VicHealth and Cancer Council Victoria. The MCCS was further augmented by Australian National Health and Medical Research Council Grants 209057, 396414 and 1074383 and by infrastructure provided by Cancer Council Victoria. Cases and their vital status were ascertained through the Victorian Cancer Registry and the Australian Institute of Health and Welfare, including the National Death Index and the Australian Cancer Database.