Glucagon-like peptide-1 control of GnRH secretion in female sheep
Leila Arbabi, Qun Li, Belinda A Henry, Iain J Clarke
JOURNAL OF ENDOCRINOLOGY | BIOSCIENTIFICA LTD | Published : 2021
The role of glucagon-like peptide-1 (GLP-1) on gonadotropin-releasing hormone (GnRH) secretion was investigated in ovariectomised (OVX) ewes, in which GnRH and luteinising hormone (LH) secretion had been restrained by treatment with oestrogen and progesterone. Guide tubes for microinjection were placed above the median eminence (ME) and the animals were allowed to recover for 1 month. Jugular venous blood samples were taken via cannulae at 10 min intervals. Vehicle (50 nL) was injected into the ME at 2 h, followed by injection of GLP-1 ((7-36)-amide - 0.5 or 1 nmol) or its receptor agonist, exendin-4 (0.5 nmol) at 4 h (n = 5). Plasma LH levels were quantified as a surrogate measure of GnRH s..View full abstract
This research was supported by a Monash University Post Graduate Scholarship awarded to Leila Arbabi.