Screening and development of monoclonal antibodies for identification of ferret T follicular helper cells
Wenbo Jiang, Julius Wong, Hyon-Xhi Tan, Hannah G Kelly, Paul G Whitney, Ian Barr, Daniel S Layton, Stephen J Kent, Adam K Wheatley, Jennifer A Juno
SCIENTIFIC REPORTS | NATURE RESEARCH | Published : 2021
The ferret is a key animal model for investigating the pathogenicity and transmissibility of important human viruses, and for the pre-clinical assessment of vaccines. However, relatively little is known about the ferret immune system, due in part to a paucity of ferret-reactive reagents. In particular, T follicular helper (Tfh) cells are critical in the generation of effective humoral responses in humans, mice and other animal models but to date it has not been possible to identify Tfh in ferrets. Here, we describe the screening and development of ferret-reactive BCL6, CXCR5 and PD-1 monoclonal antibodies. We found two commercial anti-BCL6 antibodies (clone K112-91 and clone IG191E/A8) had c..View full abstract
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Awarded by NHMRC
This work was supported by NHMRC Grants APP1149990 and GNT112909. WJ is supported by a Melbourne International Research Scholarship and Melbourne International Fee Remission Scholarship. JAJ, AKW and SJK are supported by NHMRC fellowships.