Journal article

Inhibiting the proteasome reduces molecular and biological impacts of the natural product insecticide, spinosad

Joseph Nguyen, Razi Ghazali, Philip Batterham, Trent Perry



BACKGROUND: Insecticide targets are often identified by mutations that confer resistance, but the intricacies of insecticide binding and downstream processes leading to insect death often remain obscure. Mutations in α6-like nicotinic acetylcholine receptor subunit genes have been associated with high levels of resistance to spinosad in many insect species, including Drosophila melanogaster. Here, we aimed to expand our understanding of the effects of the natural product insecticide spinosad on its protein target, the α6 subunit, using genetic tools available in D. melanogaster. RESULTS: Functional, fluorescently tagged Dα6 subunits (Dα6YFP ) were developed to allow observation of the protei..

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Awarded by Australian Research Council

Funding Acknowledgements

The authors would like to thank Dr Thomas C. Sparks and the anonymous referees for their insightful comments and suggestions on the manuscript. Imaging was conducted at the University of Melbourne, Biological Optical Microscopy Facility. The pYPet-His plasmid was a gift from Patrick Daugherty (Addgene plasmid # 14030). Bloomington Drosophila Stock Centre and the Australian Drosophila Biomedical Research Support Facility provided and imported D. melanogaster strains. Dow AgroSciences provided the D alpha 6 antibody. Funding support for this research was provided by The University of Melbourne, J.N. Peters Fellowship (TP), Australian Research Council Discovery Project scheme DP160100332 (PB) and Melbourne Research Scholarship (JN).