Journal article

The search for RNA-binding proteins: a technical and interdisciplinary challenge

Jeffrey M Smith, Jarrod J Sandow, Andrew Webb

BIOCHEMICAL SOCIETY TRANSACTIONS | PORTLAND PRESS LTD | Published : 2021

Abstract

RNA-binding proteins are customarily regarded as important facilitators of gene expression. In recent years, RNA-protein interactions have also emerged as a pervasive force in the regulation of homeostasis. The compendium of proteins with provable RNA-binding function has swelled from the hundreds to the thousands astride the partnership of mass spectrometry-based proteomics and RNA sequencing. At the foundation of these advances is the adaptation of RNA-centric capture methods that can extract bound protein that has been cross-linked in its native environment. These methods reveal snapshots in time displaying an extensive network of regulation and a wealth of data that can be used for both ..

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Grants

Funding Acknowledgements

This work was provided support by the Victorian State Government Operational Infrastructure Scheme grant and funding from an Australian Government National Health and Medical Research Council Dora Lush Postgraduate Scholarship.