Journal article

CD73( ) CD127(high) Long-Term Memory CD4 T Cells Are Highly Proliferative in Response to Recall Antigens and Are Early Targets in HIV-1 Infection

Nabila Seddiki, John Zaunders, Chan Phetsouphanh, Vedran Brezar, Yin Xu, Helen M McGuire, Michelle Bailey, Kristin McBride, Will Hey-Cunningham, Cynthia Mee Ling Munier, Laura Cook, Stephen Kent, Andrew Lloyd, Barbara Cameron, Barbara Fazekas de St Groth, Kersten Koelsch, Mark Danta, Hakim Hocini, Yves Levy, Anthony D Kelleher

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | MDPI | Published : 2021

Abstract

HIV-1 infection rapidly leads to a loss of the proliferative response of memory CD4+ T lymphocytes, when cultured with recall antigens. We report here that CD73 expression defines a subset of resting memory CD4+ T cells in peripheral blood, which highly express the α-chain of the IL-7 receptor (CD127), but not CD38 or Ki-67, yet are highly proliferative in response to mitogen and recall antigens, and to IL-7, in vitro. These cells also preferentially express CCR5 and produce IL-2. We reasoned that CD73+ memory CD4+ T cells decrease very early in HIV-1 infection. Indeed, CD73+ memory CD4+ T cells comprised a median of 7.5% (interquartile range: 4.5-10.4%) of CD4+ T cells in peripheral blood f..

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Funding Acknowledgements

This work has been supported by the Investissement d'Avenir program managed by the ANR under reference ANR-10-LABX-77 and by the Agence Nationale pour la Recherche sur le SIDA et les hepatites virales (ANRS), the Vaccine Research Institute (VRI), as well as an NHMRC Fellowship to J.Z. (ID1063422), an NHMRC Program Grant to A.D.K. (ID1052979), an NHMRC fellowship to H.M.M. (ID1037298) and an ISAC Marylou Ingram Scholars fellowship to H.M.M.