The utilisation of glutamine and glucose by a 3-D tumour model trapped in quiescence
Hannah Smith, David De Souza, Dedreia Tull, Malcolm McConville, Andrea Pellagatti, Jacqueline Boultwood, Mary Board, Richard Callaghan
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY | PERGAMON-ELSEVIER SCIENCE LTD | Published : 2021
Solid tumours modify their metabolic strategy to ensure sufficient biomass and energy to maintain a high rate of proliferation. However, solid tumours are characterised by a high proportion of quiescent cells and little is known about their metabolic profile. A tumour spheroid model with DLD1 cells was used to investigate the influence of a quiescent state on the cellular utilisation of glucose and glutamine. Quiescent DLD1 spheroids displayed increased depletion of both nutrients from the bathing medium compared to their proliferative counterparts and displayed highly active overall metabolism. A combination of biochemical and metabolomics approaches demonstrated that glucose utilisation re..View full abstract
Awarded by Medical research Council D. Phil. Studentship - NDCLS
Dr H Smith was funded by a Medical research Council D. Phil. Studentship (10CLS02JOB) awarded by NDCLS to undertake this research in the laboratory of A/Prof Callaghan. AP and JB acknowledge support from Blood Cancer UK. MB was supported by a Daphne Jackson Fellowship.